p2x7r rabbit polyclonal antibody  (Alomone Labs)

Journal: Journal of Diabetes Research

Article Title: Artificially Cultivated Ophiocordyceps sinensis Alleviates Diabetic Nephropathy and Its Podocyte Injury via Inhibiting P2X7R Expression and NLRP3 Inflammasome Activation

doi: 10.1155/2018/1390418

Figure Lengend Snippet: Primer sequences for PCR analysis in animal experiments.

Article Snippet: A rabbit anti-P2X7R polyclonal antibody (1 : 100 dilution, Alomone) or rabbit anti-NLRP3 polyclonal antibody (1 : 100 dilution, Novus) and mouse anti-synaptopodin monoclonal antibody (1 : 50 dilution, Santa Cruz) were used as primary antibodies.

Techniques: Sequencing

Journal: Journal of Diabetes Research

Article Title: Artificially Cultivated Ophiocordyceps sinensis Alleviates Diabetic Nephropathy and Its Podocyte Injury via Inhibiting P2X7R Expression and NLRP3 Inflammasome Activation

doi: 10.1155/2018/1390418

Figure Lengend Snippet: Primer sequences for PCR analysis in cellular experiments.

Article Snippet: A rabbit anti-P2X7R polyclonal antibody (1 : 100 dilution, Alomone) or rabbit anti-NLRP3 polyclonal antibody (1 : 100 dilution, Novus) and mouse anti-synaptopodin monoclonal antibody (1 : 50 dilution, Santa Cruz) were used as primary antibodies.

Techniques: Sequencing

Journal: Journal of Diabetes Research

Article Title: Artificially Cultivated Ophiocordyceps sinensis Alleviates Diabetic Nephropathy and Its Podocyte Injury via Inhibiting P2X7R Expression and NLRP3 Inflammasome Activation

doi: 10.1155/2018/1390418

Figure Lengend Snippet: Primary and secondary antibodies for Western blot assays.

Article Snippet: A rabbit anti-P2X7R polyclonal antibody (1 : 100 dilution, Alomone) or rabbit anti-NLRP3 polyclonal antibody (1 : 100 dilution, Novus) and mouse anti-synaptopodin monoclonal antibody (1 : 50 dilution, Santa Cruz) were used as primary antibodies.

Techniques: Western Blot

Journal: Journal of Diabetes Research

Article Title: Artificially Cultivated Ophiocordyceps sinensis Alleviates Diabetic Nephropathy and Its Podocyte Injury via Inhibiting P2X7R Expression and NLRP3 Inflammasome Activation

doi: 10.1155/2018/1390418

Figure Lengend Snippet: Effects of ACOS on the expression of P2X7R/NLRP3 inflammasome in the rat DN model. (a) Total RNA was extracted from renal cortex tissue, and the relative mRNA expression levels of P2X7R, NLRP3, ASC, and procaspase-1 were measured by real-time quantitative PCR. (b) Renal cortex tissue was lysed, and total lysates were analyzed by the Western blot assay with antibodies against P2X7R, NLRP3, ASC, active caspase-1 subunit P10, and β -actin, respectively. The relative protein expression level was expressed as the target protein/ β -actin ratio. Values are represented as mean ± SD. ∗ P < 0.05 and ∗∗ P < 0.01 vs. control group, # P < 0.05 and ## P < 0.01 vs. DN model group.

Article Snippet: A rabbit anti-P2X7R polyclonal antibody (1 : 100 dilution, Alomone) or rabbit anti-NLRP3 polyclonal antibody (1 : 100 dilution, Novus) and mouse anti-synaptopodin monoclonal antibody (1 : 50 dilution, Santa Cruz) were used as primary antibodies.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: Journal of Diabetes Research

Article Title: Artificially Cultivated Ophiocordyceps sinensis Alleviates Diabetic Nephropathy and Its Podocyte Injury via Inhibiting P2X7R Expression and NLRP3 Inflammasome Activation

doi: 10.1155/2018/1390418

Figure Lengend Snippet: Double immunofluorescence staining of the podocyte marker and P2X7R/NLRP3 in renal tissues of the rat DN model. The colocalization of P2X7R (a, red spots) or NLRP3 (b, red spots) and synaptopodin (a podocyte marker; green spots indicate alone localization and yellow spots indicate colocalization) in the frozen renal tissue section of the rat DN model (immunofluorescence microscopy ×400).

Article Snippet: A rabbit anti-P2X7R polyclonal antibody (1 : 100 dilution, Alomone) or rabbit anti-NLRP3 polyclonal antibody (1 : 100 dilution, Novus) and mouse anti-synaptopodin monoclonal antibody (1 : 50 dilution, Santa Cruz) were used as primary antibodies.

Techniques: Double Immunofluorescence Staining, Marker, Immunofluorescence, Microscopy

Journal: Journal of Diabetes Research

Article Title: Artificially Cultivated Ophiocordyceps sinensis Alleviates Diabetic Nephropathy and Its Podocyte Injury via Inhibiting P2X7R Expression and NLRP3 Inflammasome Activation

doi: 10.1155/2018/1390418

Figure Lengend Snippet: Effects of ACOS on the high-glucose-induced expression of P2X7R/NLRP3 inflammasome in cultured podocytes. Podocytes were incubated in medium and medium containing 30 mM glucose and/or 50 μ g/mL ACOS, respectively. (a) After 6 h of incubation, cells were harvested. Then, the total RNA was extracted, and the relative mRNA expression levels of P2X7R, NLRP3, ASC, and procaspase-1 were measured by real-time quantitative PCR. (b) After 24 h of incubation, cells were lysed and the total lysates were used to determine the protein expression levels of P2X7R, NLRP3, ASC, active caspase-1 subunit P10, and β -actin by the Western blot assay. The relative protein expression level was expressed as the target protein/ β -actin protein ratio. Values are represented as mean ± SD (n = 3). ∗ P < 0.05 and ∗∗ P < 0.01 vs. control group, # P < 0.05 and ## P < 0.01 vs. HG group.

Article Snippet: A rabbit anti-P2X7R polyclonal antibody (1 : 100 dilution, Alomone) or rabbit anti-NLRP3 polyclonal antibody (1 : 100 dilution, Novus) and mouse anti-synaptopodin monoclonal antibody (1 : 50 dilution, Santa Cruz) were used as primary antibodies.

Techniques: Expressing, Cell Culture, Incubation, Real-time Polymerase Chain Reaction, Western Blot